Protocols
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Media Recipes for Extensive Culturing
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McKay Agar Recipe
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Robotic Genomic Prep
Isolation of DNA from Samples
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PCR Amplification V3
Illumina PCR Amplification of the 16S rRNA V3 Region
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Manual Genomic Prep
Isolation of DNA from Samples
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Colony PCR
Colony PCR for Bacterial 16S Gene
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Fungi detection
PCR For Detection and Identification of Fungi in Clinical Samples
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IDseq
IDseq (Shallow 16S rRNA sequencing for for isolated colonies or small communities)
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Phenotypic Assays
Media Recipes for Phenotypic Assays
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qPCR
qPCR for Bacterial 16S rRNA Gene
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PCR V34
PCR Amplification of the 16S rRNA V34 region
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16S Read processing
Data processing for Illumina 16S reads
